At the end of this course, Students will be able to: define cell/tissue/organ culture; list the advantages and limitations of cell/tissue culture applications; describe differences between primary cultures and cell lines (finite, continuous, strains); recognize the morphology of mammalian cells in culture; list the safety equipment necessary to work in cell culture laboratories and apply the biosafety guidelines for cell culture laboratories; apply aseptic techniques and avoid common sources of contamination to the cell culture; provide the appropriate culture environment; describe the guiding principles for choosing specific culture conditions of mammalian cells; describe how to subculture mammalian cells (dissociation, concentration, counting, dilution); describe approaches to induce gene silencing and modify gene/protein expression (gene editing, siRNA, morpholinos); design cell/tissue culture-based experiments in mammalian cells; integrate cell/tissue culture-based approaches in their research projects;
devise cell/tissue culture-based approaches alternative to animal experiments in compliance with the 3R-principle.
Professor: Federica Franciosi
Class dates and times:
January 30 – 09:30 a.m – 14:00 p.m
February 02 – 13:00 p.m – 17:30 p.m
February 04 – 13:00 p.m – 17:30 p.m
February 06 – 09:30 a.m – 14:00 p.m
Course Information
- Credit: 3