At the end of this course, Students will be able to: define cell/tissue/organ culture list the advantages and limitations of cell/tissue culture applications describe differences between primary cultures and cell lines (finite, continuous, strains) recognize the morphology of mammalian cells in culture list the safety equipment necessary to work in cell culture laboratories and apply the biosafety guidelines for cell culture laboratories apply aseptic techniques and avoid common sources of contamination to the cell culture provide the appropriate culture environment and describe the guiding principles for choosing specific culture conditions of mammalian cells describe how to subculture mammalian cells (dissociation, concentration, counting, dilution) describe approaches to induce gene silencing and modify gene/protein expression (gene editing, siRNA, morpholinos) design cell/tissue culture-based experiments in mammalian cells integrate cell/tissue culture-based approaches in their research projects devise cell/tissue culture-based approaches alternative to animal experiments in compliance with the 3R-principle.
Professor: Federica Franciosi
Class dates and times:
January 29, 2025 Room L124 from 13:00 to 15:00 ; LLABBIO1 from 15:00 to 17:30
February 5, 2025 Room L124 from 13:00 to 15:00 ; LLABBIO1 15:00 to 17:30
February 7, 2025 LLABBIO1 from 13:00 to 16:00 ; Room L124 16:00 to 17:30
February 10, 2025 Room L124 from 13:00 to 17:00
Course Information
- Credit: 3