Regenerative medicine strategies for endometritis in aging reproductive mares: in vitro and in vivo studies.
The aim of the project is to understand the mechanisms of action of amniotic mesenchymal cells (AMCs) through their extracellular vesicles (EVs) in the development of endometritis by aging reproductive mares.
The project will require the isolation of equine AMCs and their EVs.
In vivo studies to evaluate the therapeutic potential of EVs for the treatment of chronic degenerative endometritis will be performed.
In vitro 3D culture of equine endometrial cell, derived from mare affected by endometritis, in presence/absence of secretome will be carried out. The efficacy of MSC-EVs in repairing injured uterine tissues will be evaluated by expression of some pro- and anti-inflammatory genes.
In-situ profiling of cell and EVs surface glycosylation patterns will be characterized to detect cell surface receptors required for EV internalization by target endometrial cells.
Analysis of proteins expressed by AMC-EVs will allow to detect proteins with a key role in the regenerative process.
Bachelor’s degree in Ricerca Biotecnologica in Medicina at Vita-Salute San Raffaele University of Milan.
Master degree in Veterinary Biotechnology at University of Milan (UNIMI).
Internship period at the Reproduction section Department of Veterinary Medicine and Animal Science (DIVAS) of Lodi.
Master thesis on amniotic extracellular vesicles and their role in the prevention of persistent post-breeding-induced endometritis.
Worked on gamete manipulation, in vitro embryo culture, culture of extra-fetal-tissue-derived mesenchymal stem cells and their application in regenerative medicine approaches.
Publications: Orcid ; IRIS-AIR
Supervisor Prof. Anna Lange Consiglio
